• Abrin
• Bacillus cereus Biovar anthracis
• Botulinum neurotoxins
• Botulinum neurotoxin producing species of Clostridium
• Conotoxins (Short, paralytic alpha conotoxins containing the following amino acid sequence X1CCX2PACGX3X4X5X6CX7)
• Coxiella burnetii
• Crimean-Congo haemorrhagic fever virus
• Diacetoxyscirpenol
• Eastern Equine Encephalitis virus
• Ebolaviruses (including Bombali, Bundibugyo, Ebola, Reston, Sudan, and Tai Forest viruses)
• Francisella tularensis
• Lassa virus
• Lujo virus
• Marburg virus
• Monkeypox virus, clade I
• Reconstructed replication-competent forms of the 1918 pandemic influenza virus containing any portion of the coding regions of all eight gene segments (Reconstructed 1918 Influenza virus)
• Ricin
• Rickettsia prowazekii
• SARS-associated coronavirus (SARS-CoV)
• SARS-CoV/SARS-CoV-2 chimeric viruses resulting from any deliberate manipulation of SARS-CoV-2 to incorporate nucleic acids coding for SARS-CoV virulence factors
• Saxitoxin
• South American Hemorrhagic Fever viruses:
  • Chapare
  • Guanarito
  • Junín
  • Machupo
• Sabia
• Staphylococcal enterotoxins (subtypes A,B,C,D,E)
• T-2 toxin
• Tetrodotoxin
• Tick-borne encephalitis complex (flavi) viruses:
  • Far-Eastern subtype
  • Siberian subtype
  • Kyasanur Forest disease virus
  • Omsk hemorrhagic fever virus
• Variola major virus (Smallpox virus)
• Variola minor virus (Alastrim)
• Yersinia pestis

Overlap Select Agents and Toxins

• Bacillus anthracis
• Bacillus anthracis Pasteur strain
• Burkholderia mallei
• Burkholderia pseudomallei
• Hendra virus
• Nipah virus
• Rift Valley fever virus (except #1 MP-12 vaccine strain)
• Venezuelan equine encephalitis virus (except TC83 and V3526 strains)

• African swine fever virus
• Avian influenza virus
• Classical swine fever virus
• Foot-and-mouth disease virus
• Goat pox virus
• Lumpy skin disease virus
• Mycoplasma capricolum
• Mycoplasma mycoides
• Newcastle disease virus
• Peste des petits ruminants virus
• Rinderpest virus
• Sheep pox virus
• Swine vesicular disease virus

• Coniothyrium glycines (formerly Phoma glycinicola and Pyrenochaeta glycines)
• Ralstonia solanacearum
• Rathayibacter toxicus
• Sclerophthora rayssiae
• Synchytrium endobioticum
• Xanthomonas oryzae

https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf

Risk Group 4 (RG4) - Bacterial Agents

• None

Risk Group 4 (RG4) - Fungal Agents

• None

Risk Group 4 (RG4) - Parasitic Agents

• None

Risk Group 4 (RG4) - Viral Agents

• Arenaviruses
  • Chapare virus
  • Guanarito virus
  • Junín virus (except the candid #1 vaccine strain listed in Appendix B-II-D Risk Group2 (RG2) – Viruses)
  • Lassa virus
  • Lujo virus
  • Machupo virus
  • Sabia
• Bunyaviruses (Nairovirus)
  • Crimean-Congo hemorrhagic fever virus
• Filoviruses
  • Ebolaviruses, including Bombali, Bundibugyo, Ebola, Reston, Sudan, and Tai Forest viruses
  • Marburg viruses, including Marburg and Ravn viruses
• Flaviruses - Group B Arboviruses
  • Tick-borne encephalitis virus complex including Absetterov, Alkhurma hemorrhagic fever, Central European encephalitis, Hanzalova, Hypr, Kumlinge, Kyasanur Forest disease, Nanjianyin, Omsk hemorrhagic fever, and Russian spring-summer encephalitis viruses
• Herpesviruses (alpha)
  • Herpesvirus simiae (Herpes B or Monkey B virus)
• Paramyxoviruses
  • Hendra virus
  • Nipah virus
• Hemorrhagic fever viruses as yet undefined

Risk Group 3 (RG3) - Bacterial Agents Including Rickettsia*

• Bartonella
• Brucella including canis
• Burkholderia (Pseudomonas) mallei, B. pseudomallei
• Coxiella burnetii (except the Phase II, Nine Mile strain listed in Appendix B-II-A, Risk Group 2 (RG2) - Bacterial Agents Including Chlamydia)
• Francisella tularensis (except those strains listed in Appendix B-II-A, Risk Group 2 (RG2) – Bacterial Agents Including Chlamydia)
• Orientia tsutsugamushi (was tsutsugamushi)
• Pasteurella multocida type B -"buffalo" and other virulent strains
• Rickettsia akari, R. australis, R. canada, R. conorii, R. prowazekii, R. rickettsii, R. siberica, R. typhi ( mooseri)
• Yersinia pestis (except those strains listed in Appendix B-II-A, Risk Group 2 (RG2) - Bacterial Agents Including Chlamydia)

Risk Group 3 (RG3) - Fungal Agents*

• None

Risk Group 3 (RG3) - Parasitic Agents

• None

Risk Group 3 (RG3) - Viruses and Prions*

• Alphaviruses (Togaviruses) - Group A Arboviruses
  • Chikungunya virus (except the vaccine strain 181/25 listed in Appendix B-II-D Risk Group2 (RG2) – Viruses)
  • Semliki Forest virus
  • Venezuelan equine encephalomyelitis virus (except the vaccine strains TC-83 and V3526, see Appendix B-II-D (RG2) – Viruses)
  • Other viruses as listed in the reference source (see Section V-C, Footnotes and References of Sections I through IV)
• Arenaviruses
  • Flexal
  • Lymphocytic choriomeningitis virus (LCM) (neurotropic strains)
• Bunyaviruses
  • Hantaviruses, including Hantaan virus
  • Rift Valley fever virus (except #1 MP-12)
• Coronaviruses
  • SARS-associated coronavirus (SARS-CoV)
  • Middle East respiratory syndrome coronavirus (MERS-CoV)
• Flaviviruses - Group B Arboviruses
  • Japanese encephalitis virus (except those strains listed in Appendix B-II-D Risk Group2 (RG2) - Viruses)
  • Yellow fever virus (except 17-D)
  • Other viruses as listed in the reference source (see Section V-C, Footnotes and References of Sections I through IV)
• Orthomyxoviruses
  • Influenza viruses 1918-1919 H1N1 (1918 H1N1), human H2N2 (1957-1968), and highly pathogenic avian influenza H5N1 strains within the Goose/Guangdong/96-like H5 lineage (HPAI H5N1).
• Poxviruses
  • Monkeypox virus (Clade I & Clade II containing nucleic acids coding for clade I MPVX virus virulence factors)
• Prions
  • Transmissible spongiform encephalopathies (TSE) agents (Creutzfeldt-Jacob disease and kuru agents)(see Section V-C, Footnotes and References of Sections I through IV, for containment instruction)

• Human immunodeficiency virus (HIV) types 1 and 2
• Human T cell lymphotropic virus (HTLV) types 1 and 2
• Simian immunodeficiency virus (SIV)
• Mycobacterium tuberculosis, Mycobacterium bovis
• Clade II of MPVX viruses unless containing nucleic acids coding for clade I MPVX virus virulence factors
• Vesicular stomatitis virus
• Coccidioides immitis and Coccidioides posadasii (sporulating cultures; contaminated soil)
• Sporulating cultures of Histoplasma capsulatum, capsulatum var. Duboisii
• Sporulating cultures of Blastomyces (e.g., B. dermatitidis, B. gilchristii)